Exogenous supply of glutamine and active cytokinin to the roots reduces NO₃^- uptake rates in poplar (2024)

Dluzniewska, P., Gessler, A., Kopriva, S., Strnad, M., Novák, O., Dietrich, H., & Rennenberg, H. (2006). Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar. Plant, Cell and Environment, 29(7), 1284-1297. https://doi.org/10.1111/j.1365-3040.2006.01507.x

Dluzniewska, Paulina ; Gessler, Arthur ; Kopriva, Stanislav et al. / Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar. In: Plant, Cell and Environment. 2006 ; Vol. 29, No. 7. pp. 1284-1297.

@article{71c9d0e7cc8e4c27aec2db847d3c4e26,

title = "Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar",

abstract = "The present study shows for the first time the influence of exogenously applied amino acids and cytokinin on the physiological and molecular aspects of N metabolism in poplar trees. In a short-term feeding experiment, glutamine or trans-zeatin riboside (tZR) was added directly to the nutrient solution. NO 3- net uptake declined significantly in response to both treatments. Feeding with glutamine brought about an increase in concentrations of different amino compounds in the roots (glutamine, glutamate, alanine, γ-amino butyric acid (GABA) and NH4+, which negatively correlated with the net NO3- uptake. The plants showed a reduction of cytosolic glutamine synthetase 1 (GS1) transcript level in the roots. In addition, glutamine feeding changed the root-to-shoot distribution on N assimilation in favour of the leaves and plant internal N cycling. tZR treatment resulted in expansion of zeatin-type (Z-type) cytokinins in the roots and increased nitrate reductase (NR)-mRNA level. The results indicate that both particular amino acids and active cytokinins are involved in the feedback regulation of N uptake and metabolism in poplar. We propose that inhibition of N uptake by cytokinins in poplar is more complex than that mediated by amino compounds, and other effectors are involved in this regulation.",

keywords = "Amino acids, N cycling, Trans-zeatin riboside",

author = "Paulina Dluzniewska and Arthur Gessler and Stanislav Kopriva and Miroslav Strnad and Ond{\v r}ej Nov{\'a}k and Henriette Dietrich and Heinz Rennenberg",

year = "2006",

month = jul,

doi = "10.1111/j.1365-3040.2006.01507.x",

language = "English",

volume = "29",

pages = "1284--1297",

journal = "Plant, Cell and Environment",

issn = "0140-7791",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "7",

}

Dluzniewska, P, Gessler, A, Kopriva, S, Strnad, M, Novák, O, Dietrich, H & Rennenberg, H 2006, 'Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar', Plant, Cell and Environment, vol. 29, no. 7, pp. 1284-1297. https://doi.org/10.1111/j.1365-3040.2006.01507.x

Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar. / Dluzniewska, Paulina; Gessler, Arthur; Kopriva, Stanislav et al.
In: Plant, Cell and Environment, Vol. 29, No. 7, 07.2006, p. 1284-1297.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar

AU - Dluzniewska, Paulina

AU - Gessler, Arthur

AU - Kopriva, Stanislav

AU - Strnad, Miroslav

AU - Novák, Ondřej

AU - Dietrich, Henriette

AU - Rennenberg, Heinz

PY - 2006/7

Y1 - 2006/7

N2 - The present study shows for the first time the influence of exogenously applied amino acids and cytokinin on the physiological and molecular aspects of N metabolism in poplar trees. In a short-term feeding experiment, glutamine or trans-zeatin riboside (tZR) was added directly to the nutrient solution. NO 3- net uptake declined significantly in response to both treatments. Feeding with glutamine brought about an increase in concentrations of different amino compounds in the roots (glutamine, glutamate, alanine, γ-amino butyric acid (GABA) and NH4+, which negatively correlated with the net NO3- uptake. The plants showed a reduction of cytosolic glutamine synthetase 1 (GS1) transcript level in the roots. In addition, glutamine feeding changed the root-to-shoot distribution on N assimilation in favour of the leaves and plant internal N cycling. tZR treatment resulted in expansion of zeatin-type (Z-type) cytokinins in the roots and increased nitrate reductase (NR)-mRNA level. The results indicate that both particular amino acids and active cytokinins are involved in the feedback regulation of N uptake and metabolism in poplar. We propose that inhibition of N uptake by cytokinins in poplar is more complex than that mediated by amino compounds, and other effectors are involved in this regulation.

AB - The present study shows for the first time the influence of exogenously applied amino acids and cytokinin on the physiological and molecular aspects of N metabolism in poplar trees. In a short-term feeding experiment, glutamine or trans-zeatin riboside (tZR) was added directly to the nutrient solution. NO 3- net uptake declined significantly in response to both treatments. Feeding with glutamine brought about an increase in concentrations of different amino compounds in the roots (glutamine, glutamate, alanine, γ-amino butyric acid (GABA) and NH4+, which negatively correlated with the net NO3- uptake. The plants showed a reduction of cytosolic glutamine synthetase 1 (GS1) transcript level in the roots. In addition, glutamine feeding changed the root-to-shoot distribution on N assimilation in favour of the leaves and plant internal N cycling. tZR treatment resulted in expansion of zeatin-type (Z-type) cytokinins in the roots and increased nitrate reductase (NR)-mRNA level. The results indicate that both particular amino acids and active cytokinins are involved in the feedback regulation of N uptake and metabolism in poplar. We propose that inhibition of N uptake by cytokinins in poplar is more complex than that mediated by amino compounds, and other effectors are involved in this regulation.

KW - Amino acids

KW - N cycling

KW - Trans-zeatin riboside

UR - http://www.scopus.com/inward/record.url?scp=33744949616&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3040.2006.01507.x

DO - 10.1111/j.1365-3040.2006.01507.x

M3 - Article

SN - 0140-7791

VL - 29

SP - 1284

EP - 1297

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

IS - 7

ER -

Dluzniewska P, Gessler A, Kopriva S, Strnad M, Novák O, Dietrich H et al. Exogenous supply of glutamine and active cytokinin to the roots reduces NO3- uptake rates in poplar. Plant, Cell and Environment. 2006 Jul;29(7):1284-1297. doi: 10.1111/j.1365-3040.2006.01507.x