Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms (2024)

Nakagawa, H., Jiang, C. J., Sakakibara, H., Kojima, M., Honda, I., Ajisaka, H., Nishijima, T., Koshioka, M., Homma, T., Mander, L. N., & Takatsuji, H. (2005). Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms. Plant Journal, 41(4), 512-523. https://doi.org/10.1111/j.1365-313X.2004.02316.x

Nakagawa, Hitoshi ; Jiang, Chang Jie ; Sakakibara, Hitoshi et al. / Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms. In: Plant Journal. 2005 ; Vol. 41, No. 4. pp. 512-523.

@article{2a98979c8e514ab092faacaa275ecead,

title = "Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms",

abstract = "Shoot branching and plant height are among the key factors that define the overall architecture of plants. We found that overexpression of a cDNA for a zinc-finger protein of petunia, designated Lateral shoot-Inducing Factor (LIF), in transgenic petunia plants resulted in a dramatic increase in lateral shoots and reduced plant height. LIF overexpression also caused a decrease in the number of cells in the stem, leaf, and flower, accompanied by enlargement of cells. trans-Zeatin was decreased while N6-(Δ2- isopentenyl)adenine was increased in the leaves of LIF-overexpressed petunia. Most of the riboside, ribotide, and glucoside forms were also increased. Expression analysis using a LIF::GUSfusion gene and RT-PCR suggested that LIF is specifically expressed around the bases of axillary buds and weakly in basal part of flowers in wild-type petunia. GFP-LIF-GUS fusion proteins were translocated into the nucleus when transiently expressed in onion epidermal cells. LIF overexpression resulted in enhanced branching also in tobacco and Arabidopsis, indicating the conservation of the response to LIF overexpression among dicotyledonous plants. On the basis of these results we discuss about possible functions of LIF.",

keywords = "Arabidopsis, Axillary branching, Cytokinin, Petunia, Tobacco, Transcription factor",

author = "Hitoshi Nakagawa and Jiang, {Chang Jie} and Hitoshi Sakakibara and Mikiko Kojima and Ichiro Honda and Hidetoshi Ajisaka and Takaaki Nishijima and Masaji Koshioka and Tamaki Homma and Mander, {Lewis N.} and Hiroshi Takatsuji",

year = "2005",

month = feb,

doi = "10.1111/j.1365-313X.2004.02316.x",

language = "English",

volume = "41",

pages = "512--523",

journal = "Plant Journal",

issn = "0960-7412",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "4",

}

Nakagawa, H, Jiang, CJ, Sakakibara, H, Kojima, M, Honda, I, Ajisaka, H, Nishijima, T, Koshioka, M, Homma, T, Mander, LN & Takatsuji, H 2005, 'Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms', Plant Journal, vol. 41, no. 4, pp. 512-523. https://doi.org/10.1111/j.1365-313X.2004.02316.x

Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms. / Nakagawa, Hitoshi; Jiang, Chang Jie; Sakakibara, Hitoshi et al.
In: Plant Journal, Vol. 41, No. 4, 02.2005, p. 512-523.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms

AU - Nakagawa, Hitoshi

AU - Jiang, Chang Jie

AU - Sakakibara, Hitoshi

AU - Kojima, Mikiko

AU - Honda, Ichiro

AU - Ajisaka, Hidetoshi

AU - Nishijima, Takaaki

AU - Koshioka, Masaji

AU - Homma, Tamaki

AU - Mander, Lewis N.

AU - Takatsuji, Hiroshi

PY - 2005/2

Y1 - 2005/2

N2 - Shoot branching and plant height are among the key factors that define the overall architecture of plants. We found that overexpression of a cDNA for a zinc-finger protein of petunia, designated Lateral shoot-Inducing Factor (LIF), in transgenic petunia plants resulted in a dramatic increase in lateral shoots and reduced plant height. LIF overexpression also caused a decrease in the number of cells in the stem, leaf, and flower, accompanied by enlargement of cells. trans-Zeatin was decreased while N6-(Δ2- isopentenyl)adenine was increased in the leaves of LIF-overexpressed petunia. Most of the riboside, ribotide, and glucoside forms were also increased. Expression analysis using a LIF::GUSfusion gene and RT-PCR suggested that LIF is specifically expressed around the bases of axillary buds and weakly in basal part of flowers in wild-type petunia. GFP-LIF-GUS fusion proteins were translocated into the nucleus when transiently expressed in onion epidermal cells. LIF overexpression resulted in enhanced branching also in tobacco and Arabidopsis, indicating the conservation of the response to LIF overexpression among dicotyledonous plants. On the basis of these results we discuss about possible functions of LIF.

AB - Shoot branching and plant height are among the key factors that define the overall architecture of plants. We found that overexpression of a cDNA for a zinc-finger protein of petunia, designated Lateral shoot-Inducing Factor (LIF), in transgenic petunia plants resulted in a dramatic increase in lateral shoots and reduced plant height. LIF overexpression also caused a decrease in the number of cells in the stem, leaf, and flower, accompanied by enlargement of cells. trans-Zeatin was decreased while N6-(Δ2- isopentenyl)adenine was increased in the leaves of LIF-overexpressed petunia. Most of the riboside, ribotide, and glucoside forms were also increased. Expression analysis using a LIF::GUSfusion gene and RT-PCR suggested that LIF is specifically expressed around the bases of axillary buds and weakly in basal part of flowers in wild-type petunia. GFP-LIF-GUS fusion proteins were translocated into the nucleus when transiently expressed in onion epidermal cells. LIF overexpression resulted in enhanced branching also in tobacco and Arabidopsis, indicating the conservation of the response to LIF overexpression among dicotyledonous plants. On the basis of these results we discuss about possible functions of LIF.

KW - Arabidopsis

KW - Axillary branching

KW - Cytokinin

KW - Petunia

KW - Tobacco

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=20044390548&partnerID=8YFLogxK

U2 - 10.1111/j.1365-313X.2004.02316.x

DO - 10.1111/j.1365-313X.2004.02316.x

M3 - Article

SN - 0960-7412

VL - 41

SP - 512

EP - 523

JO - Plant Journal

JF - Plant Journal

IS - 4

ER -

Nakagawa H, Jiang CJ, Sakakibara H, Kojima M, Honda I, Ajisaka H et al. Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms. Plant Journal. 2005 Feb;41(4):512-523. doi: 10.1111/j.1365-313X.2004.02316.x